propidium iodide (MedChemExpress)

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MedChemExpress propidium iodide
Propidium Iodide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide/Propidium/pm42521400-122-22-25
Average 98 stars, based on 265 article reviews

propidium iodide - by Bioz Stars, 2026-10

98/100 stars

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Staining:

Article Title: Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer
Article Snippet: Next, RNA is digested by incubating cells with 500 μL RNase A (20 μg/mL, EN0531, Thermo Scientific, Waltham, MA, USA) at room temperature for 30 min. .. The cells are then stained with propidium iodide (PI, 50 μg/mL, HY-D0815, MedChemExpress, Shanghai, China) for 30 min to label DNA. .. Finally, the cell cycle distribution is analyzed using a flow cytometer (FACSVerse, BD Biosciences, San Jose, CA, USA) to determine the proportion of cells in each phase.

Article Title: Crocin Targets HSP90 to Suppress NLRP3/Caspase-1-Mediated Sertoli Cell Pyroptosis.
Article Snippet: Pyroptosis, a proinflammatory form of programmed cell death mediated by the NLRP3 inflammasome, has recently been implicated in Sertoli cell inflammatory injury.. Crocin, a water‐soluble carotenoid glycoside derived from Crocus sativus L. and Gardenia jasminoides Ellis, exhibits anti‐inflammatory, antioxidant, and anti‐apoptotic properties, yet its role in Sertoli cell pyroptosis remains unclear.. Here, we investigated the pharmacological effects and mechanisms of crocin in regulating TM4 Sertoli cell viability and pyroptosis.

Article Title: A combinatorial therapeutic platform based on graphene-collagen cryogel and apoptotic vesicles for modulating hypertrophic scars.
Article Snippet: As for flow cytometry, ASCs were harvested through trypsinization (37°C, 2-3min) after 12h STS treatment, washed twice with ice-cold PBS, and subsequently resuspended in 1× Annexin V binding buffer (Sigma-Aldrich, APOAF-20TST) at a standardized density of 1×10^6 cells/mL for subsequent apoptosis analysis. .. Cell suspensions were then stained with 5 μL Annexin VFITC (488 nm excitation/530 nm emission) and 5 μL propidium iodide (PI, 488 nm excitation/575 nm emission) for 15 minutes at room temperature in the dark. (4) Absorption of ASCs-ApoVs by HS-fibroblasts: a.DiO labeling of ASCs-ApoVs: ASCs were incubated with 5-10 μM DiO (MCE, HY-D0969; λex/λ em = 484/501 nm) in culture medium for 20 min at 37 °C, washed, and then subjected to apoptosis induction (5 μM STS, 12 h) to generate DiO-labeled ASCs-ApoVs. b. CM-Dil labeling of HS-fibroblasts for subcellular localization: HS-fibroblasts’ membranous organelle was stained with CM-Dil (InvitrogenTM, C7000; λex/λem = 553/570 nm) by incubating with 5 μL of labeling solution per 1 mL growth medium for 20 min at 37 °C, followed by washing. c. Cellular uptake assay: DiO-labeled ASCs-ApoVs were co-cultured with CM-Dil-pre-labeled HS-fibroblasts for 2 h at 37 ° C. Nuclei were then counterstained with Hoechst 33342 (MCE, HY-15559; λex/λem = 346/460 nm) for 15 min at room temperature. ..

Article Title: Spermidine-functionalized Janus hydrogel microneedles inhibit ferroptosis and promote healing of oral ulcers
Article Snippet: .. Assessment of cellular demise was performed by staining with propidium iodide (PI, HY-D0815, MCE). ..

Article Title: Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer.
Article Snippet: Next, RNA is digested by incubating cells with 500 μL RNase A (20 μg/mL, EN0531, Thermo Scientific, Waltham, MA, USA) at room temperature for 30 minutes. .. The cells are then stained with propidium iodide (PI, 50 μg/mL, HY-D0815, MedChemExpress, Shanghai, China) for 30 minutes to label DNA. .. Finally, the cell cycle distribution is analyzed using a flow AR TI CL E IN P RE SS cytometer (FACSVerse, BD Biosciences, San Jose, CA, USA) to determine the proportion of cells in each phase.

Blocking Assay:

Article Title: Tyrosine-protein phosphatase non-receptor type 2 deficiency in macrophages diminishes the anti-inflammatory and cardioprotective effects of gabapentin after myocardial infarction.
Article Snippet: Funding information Natural Science Foundation of Heilongjiang Province, Grant/Award Number: JJ2020LH0293 Background and Purpose: Our previous study has highlighted the anti-inflammatory properties of gabapentin (GBP) after myocardial infarction (MI) by suppressing M1 macrophage polarization.. In this study, we further dissected the important molecules involved.. Experimental Approach: Ligation of the left anterior descending coronary artery was used to construct the MI mouse model. Tyrosine-protein phosphatase non-receptor type 2 (PTPN2) expression in mouse myocardial tissue macrophages was evaluated using Western blot and dual-labelling immunofluorescence.

FACS:

Incubation:

Article Title: HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replication
Article Snippet: .. Treated cells were incubated with 1x Hoechst 33342 (Invitrogen) and 500 nM Propidium Iodide (MedChemExpress) for 60 min at 37°C. ..

Labeling:

Stability:

Article Title: Transient perinuclear actin rings prevent cell aging and apoptosis via nuclear mechanical protection.
Article Snippet: Epithelial tissues in vivo are often subjected to the challenge of mechanical forces and osmotic shock.. However, how epithelial cells prevent DNA damage and reduce aging and apoptosis in the presence of these intense external stimuli remains unclear.. Here, we found a reversible perinuclear actin ring can be assembled after rapid and significant hypotonic shock or mechanical forces.

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